Plasma & Blood Protein Binding Assay

• Compounds may bind to plasma proteins so only a small fraction of the drug is free in the plasma
• Rapid equilibrium dialysis
• Cross-species plasma protein binding
• Results reported as % bound and fraction unbound (fu)

Cell Culture Media Binding Assays

• Compounds may bind to serum proteins in cell culture media so that only a small fraction of the drug is actually free in the media
• Rapid equilibrium dialysis
• A diverse selection of media available
• Results reported as % bound and fraction unbound (fu)

Brain Tissue Binding Assays

• Compounds may bind to brain proteins and lipids so only a small fraction of the drug is free in the plasma
• Different to plasma protein binding as plasma has twice as much protein and brain has 20-fold more lipids
• Rapid equilibrium dialysis method
• Cross-species brain tissue binding
• Results reported as % bound and fraction unbound (fu)

Tissue Binding Assays

• Compounds may bind to tissue proteins and lipids so only a small fraction of the drug is free in the plasma
• Rapid equilibrium dialysis method
• A wide range of tissues and organs available
• Cross-species tissue binding
• Results reported as % bound and fraction unbound (fu)