Plasma & Blood Protein Binding Assay
- Compounds may bind to plasma proteins so only a small fraction of the drug is free in the plasma
- Rapid equilibrium dialysis
- Cross-species plasma protein binding
- Results reported as % bound and fraction unbound (fu)
Cell Culture Media Binding Assays
- Compounds may bind to serum proteins in cell culture media so that only a small fraction of the drug is actually free in the media
- Rapid equilibrium dialysis
- A diverse selection of media available
- Results reported as % bound and fraction unbound (fu)
Brain Tissue Binding Assays
- Compounds may bind to brain proteins and lipids so only a small fraction of the drug is free in the plasma
- Different to plasma protein binding as plasma has twice as much protein and brain has 20-fold more lipids
- Rapid equilibrium dialysis method
- Cross-species brain tissue binding
- Results reported as % bound and fraction unbound (fu)
Tissue Binding Assays
- Compounds may bind to tissue proteins and lipids so only a small fraction of the drug is free in the plasma
- Rapid equilibrium dialysis method
- A wide range of tissues and organs available
- Cross-species tissue binding
- Results reported as % bound and fraction unbound (fu)