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Thermodynamic Solubility Assays


  • Thermodynamic solubility tests evaluate the solubility of solid crystalline material in aqueous solvent as a saturated solution in equilibrium
  • Thermodynamic aqueous solubility tests can be performed in any media or buffer


Kinetic Solubility Assays


  • Aqueous kinetic solubility tests evaluate the solubility of a compound already fully dissolved in an organic solvent
  • Kinetic solubility tests can be performed in any media or buffer


Fasted State Simulated Gastric Fluid (FaSSGF) pH 1.6 Solubility


  • Solubility assay performed in artificial FaSSGF
  • This biologically relevant fluid mimics gastric juices because it contains natural surfactants (salts and acids)


Fasted (FaSSIF) and Fed State Simulated Intestinal Fluid (FeSSIF) pH 6.5 Solubility Assays


  • Solubility assay performed in artificial FaSSIF/FeSSIF
  • This biologically relevant fluid mimics intestinal juice because it contains natural surfactants (bile salts and lecithin)


Partition Coefficient (Log D) Assays


  • Log D coefficient is the ratio of concentrations of a compound in the two phases of a mixture of two immiscible solvents at equilibrium
  • This method involves dissolving the compound in a known volume of octanol (hydrophobic) and water or PBS (hydrophilic)
  • Shake-flask method
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